生物测定系统/QuantiChrom&trade；洗涤剂分析试剂盒/100试验/DDTR-100
商品编号：
DDTR-100
品牌：
bioassaysys
市场价：
¥7180.00
美元价：
3590.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Detergent Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Protein Purification: Traces of detergent in purified protein samples.
- Environment: Detergent determination in water and soil.
Key Features
- Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.
- Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm.
- Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.
Method
- 560nm and 650nm
Samples
- Biological and environmental samples
Species
- All
Size
- 100 tests
Detection Limit
- Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.
Shelf Life
- 12 Months
More Details
- Detergents are surfactants that are amphiphilic, which means that they are partly hydrophobic and partly hydrophilic. Detergents fall into three categories: anionic, cationic, and non-ionic/zwitterionic. They are commonly used in household cleaning products, in gasoline as additives, and in biological reagents. Simple, direct and automation-ready procedures for measuring detergent concentration in biological samples are very desirable. BioAssay Systems" detergent assay kit is designed to measure detergent directly without any pretreatment. Above the critical micellar concentration, detergent forms micelle in solution that trap the colorimetric dye. The intensity of the color is directly proportional to the detergent concentration in the sample.
What samples have been tested with this assay?
This kit has been tested with several varieties of handsoap, dish detergent, and powder clothing detergent.You haven’t tested the detergent I am interested in measuring. Is my detergent compatible with the kit?
Since we haven’t tested every single detergent possible with the kit, there may be some which are not compatible. However, as long as it is not a bile acid it is likely to work. We have found that most lower detection limits tend to be the critical micellar concentration of the detergent (CMC) so if the detergent is compatible, the lower detection limit is probably the CMC. You should construct your own standard curve if you have a purified stock of the detergent, and can determine the concentration using this curve.Can I use this assay with wastewater?
This assay has not been tested with wastewater. However, Dye B is commonly used to test wastewater for detergents although it requires several purification and extraction steps with a separatory funnel to remove interference. See https://doi.org/10.1002/j.1551-8833.1975.tb02144.x for more information.Can I use this kit on a sample with multiple detergents in it?
If one detergent is anionic and another is cationic, you may. However, if both detergents are the same charge or are nonionic then they will both be measured by the assay and the true concentration of each cannot be determined. Thus it gives an overall detergent strength.The OD of my detergent is linear for lower concentrations, then drops, then increases again. Why?
Yes, some detergents behave this way. It is recommended to create a standard curve of detergent that is linear and exclude the higher concentrations that are not linear.My sample is cloudy. What is causing this interference with this assay?
Sodium deoxycholate and other bile acids precipitate out of solution with this assay and are cloudy, so cannot be used. Additionally, high concentrations of compatible detergents can cause cloudiness, so samples must be diluted to within detection limits.My sample is colored, will this interfere with the assay?
You can run a background of 20 µL sample + 180 µL DI water to see if there is significant absorbance at OD560 or OD650 nm, and subtract this value from your sample when used with working reagent. You may also have to dilute your sample if detergent concentrations are too high, and this may help with reducing color.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
