生物测定系统/酶染色与贸易；GSH/GSSG分析试剂盒/100试验/EGTT-100
商品编号：
EGTT-100
品牌：
bioassaysys
市场价：
¥5780.00
美元价：
2890.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ GSH/GSSG Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of reduced and oxidized glutathione (GSH/GSSG).
Key Features
- Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.
Method
- OD412nm
Samples
- Whole blood, plasma, serum, urine, tissue and cell extracts
Species
- All
Size
- 100 tests
Detection Limit
- 10 nM
Shelf Life
- 6 months
More Details
- Glutathione , a tripeptide of glycine, glutamic acid and cysteine, is one of the key antioxidants involved in protecting cells from damages by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density,measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Lin, T. A., Ke, B. J., Cheng, C. S., Wang, J. J., Wei, B. L., & Lee, C. L. (2019). Red Quinoa Bran Extracts Protects against Carbon Tetrachloride-Induced Liver Injury and Fibrosis in Mice via Activation of Antioxidative Enzyme Systems and Blocking TGF-beta1 Pathway. Nutrients, 11(2), 395. Assay: GSH/GSSG in mice tissues. Kim, K. H., Kim, E. Y., Lee, S. Y., Ko, J. J., & Lee, K. A. (2018). Oocyte Cytoplasmic Gas6 and Heparan Sulfate (HS) are Required to Establish the Open Chromatin State in Nuclei During Remodeling and Reprogramming. Cellular Physiology and Biochemistry, 45(1), 37-53. Assay: GSH/GSSG in mice cells. Li, X. X., Wang, Z. J., Zheng, Y., Guan, Y. F., Yang, P. B., Chen, X. & Chien, K. Y. (2018). Nuclear receptor Nur77 facilitates melanoma cell survival under metabolic stress by protecting fatty acid oxidation. Molecular cell, 69(3), 480-492. Assay: GSH/GSSG in mice cells. Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: GSH/GSSG in D. melanogaster tissues.Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2018). STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox biology, 14, 423-438. Assay: GSH/GSSG in rat tissues and plasma. Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: GSH/GSSG in human tissues. Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2017). Deciphering Molecular Cascades In A Novel Acclimatization Strategy For Rapid Ascent To High Altitude. bioRxiv, 145342. Assay: GSH/GSSG in rat tissues and plasma.Khatri, R., Krishnan, S., Roy, S., Chattopadhyay, S., Kumar, V., & Mukhopadhyay, A. (2016). Reactive oxygen species limit the ability of bone marrow stromal cells to support hematopoietic reconstitution in aging mice. Stem cells and development 25(12):948-58. Assay: GSH/GSSG in mice stromal cells. Lee, H. N., et al. (2015). Heme Oxygenase-1 Determines the Differential Response of Breast Cancer and Normal Cells to Piperlongumine. Molecules and cells, 38(4), 327. Assay: GSH/GSSG in piperlongumine cell. Chatterjee, N., Eom, H. J., & Choi, J. (2014). A systems toxicology approach to the surface functionality control of graphene-cell interactions. Biomaterials, 35(4), 1109-1127. Assay: GSH/GSSG in human HepG2 cells. Di Maro, G., et al. (2014). Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells. Molecular cancer, 13(1), 1. Assay: GSH/GSSG in human tissues. Muller, S., et al. (2014). Age-dependent effects of UCP2 deficiency on experimental acute pancreatitis in mice. PloS one, 9(4), e94494. Assay: GSH/GSSG in mice serum.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
