生物测定系统/QuantiChrom&trade；羰基分析试剂盒/100试验/DCAR-100
商品编号：
DCAR-100
品牌：
bioassaysys
市场价：
¥5980.00
美元价：
2990.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Carbonyl Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Quantification of carbonyl groups or protein carbonyls in biological samples.
Key Features
- Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD375nm
Samples
- Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc.)
Species
- All
Size
- 100 tests
Detection Limit
- 12 µM
Shelf Life
- 12 months
More Details
- CARBONYL groups, such as ketones and aldehydes, are a commonindicator of protein oxidation. Protein oxidation is caused as a result of exposure to reactive oxygen species (ROS) and is a common marker in various diseases as well as aging. BioAssay Systems' Carbonyl Assay Kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.
The background is very high. Is this normal?
The background absorbance is typically ~ 0.5. This is completely normal.Can I store the diluted standards (250 µM, 150 µM, 75 µM, and 0 µM) to use for later?
We recommend preparing the standards fresh from the 50 mM each day.Do I need to run a standard curve with every assay?
Yes.How should I store the kit?
The kit is shipped at room temperature. Upon delivery, store at -20°C.Precipitate formed when I added the reagent to the sample. What should I do?
The Reagent is acidic and may cause precipitation in samples that have significant amounts of protein. You may need to dilute your sample, repeat the assay, and correct for the dilution factor in the calculation.What is the Carbonyl Standard?
The standard is pyruvate, which has two carbonyl groups per molecule. So the 50 mM Carbonyl Standard is actually 25 mM pyruvate.My sample has high levels of nucleic acids. Will this interfere?
Nucleic acids can artificially increase the carbonyl count if they are present in high levels. If the ratio of 280/260 nm is less than 1, samples should be mixed with 1% streptomycin sulfate to denature the nucleic acids. Mix well, incubate the samples at room temperature for 15 minutes and then centrifuge at 5000xg for 10 minutes. Use the supernatant in the assay.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
