生物测定系统/enzyflou&trade；乙醛检测试剂盒/100试验/EFAC-100
商品编号：
EFAC-100
品牌：
bioassaysys
市场价：
¥5380.00
美元价：
2690.00
产品分类：
表面活性剂
公司分类：
surfactant
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyFluo™ Acetaldehyde Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Acetaldehyde determination in biological and food/beverage samples.
Key Features
- Fast and sensitive. Linear detection range (50µL sample): 0.5 µMto 60 µMacetaldehyde in 96-well plate assay.
- High-throughput.The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
- Convenient.Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- FL530/585nm
Samples
- Biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).
Species
- All
Size
- 100 tests
Detection Limit
- 0.5 µM
Shelf Life
- 6 months
More Details
- ACETALDEHYDE (CH3CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing often referred to as “Asian flush syndrome.” Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine. BioAssay Systems colorimetric acetaldehyde assay kit is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the formed NADH reduces a formazan reagent. The intensity of the product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.
How specific is this assay for acetaldehyde?
The assay mechanism uses the enzyme aldehyde dehydrogenase to quantify acetaldehyde in the sample. The enzyme will also oxidize other aldehydes in the sample (i.e. propionaldehyde). Therefore, the assay will not distinguish between acetaldehyde and other aldehydes present in the sample. Consideration should be taken if high levels of other aldehydes are expected in the sample being tested.How long are the diluted standards good for?
Acetaldehyde is a rather volatile chemical, so proper storage of the standard is vital. Only bring the 3 M Standard to room temperature for time needed to prepare standards. Return to -20°C within 30 minutes of thawing. Be sure to keep the Standard and standard dilutions on ice or refrigerated with the tube caps closed during assay procedure. The diluted standards for the colorimetric assay (4 mM, 2.4 mM, 1.2 mM, and 0 mM) and the fluorimetric assay (60 µM, 36 µM, 18 µM, and 0 µM) should be prepared fresh before each assay and used within 10 minutes of preparing.What temperature do I run the assay at?
The assay may be run at room temperature (25°C) or 37°C.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
