生物测定系统/QuantiChrom&trade；G6PDH抑制剂筛选试剂盒/100试验/IGPDH-100
商品编号：
IGPDH-100
品牌：
bioassaysys
市场价：
¥6780.00
美元价：
3390.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ G6PDH Inhibitor Screening Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- HTS for inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.
Key Features
- Safe. Non-radioactive assay.
- High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system.
- Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed.
Method
- OD565nm
Samples
- Glucose-6-phosphate dehydrogenase
Species
- All
Size
- 100 tests
Shelf Life
- 6 months
More Details
- GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics. BioAssay Systems non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.
Does the kit come with the G6PDH enzymes or control inhibitors?
No, these are not included.How do I store the kit?
Store all components at -20°C for long term storage.What species of G6PDH was the kit optimized for?
The kit was optimized for L. Mesenteroides G6PDH.What if I am interested in testing inhibition of a species besides L. Mesenteroides?
We recommend that you experimentally determine the Km and then adjust the volume of substrate so that the final concentration of the substrate in the 100 µL Reaction Volume is near the Km.What G6PDH inhibitor was tested using this kit?
Zn(NO3)2 in dH20.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
