生物测定系统/酶染色与贸易；神经氨酸酶检测试剂盒/100次检测/ENEU-100
商品编号：
ENEU-100
品牌：
bioassaysys
市场价：
¥9180.00
美元价：
4590.00
产品分类：
组织标本
公司分类：
Tissue_samples
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Neuraminidase Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of neuraminidase activity and screen for neuraminidase inhibitor.
Key Features
- Sensitive and accurate. Linear detection range at 37°C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays.
- Simple and convenient. Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay to screen thousands of samples per day.
Method
- OD570nm, or FL530/585nm
Samples
- Biological
Species
- All
Size
- 100 tests
Detection Limit
- OD, FL: 1, 0.01U/L
Shelf Life
- 6 months
More Details
- NEURAMINDASE (also known as Sialidase) is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery. BioAssay Systems neuraminidase assay measures the sialic acid released by neuraminidase in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to neuraminidase activity in the sample.
Could you tell me the chemical components of the substrate? What kind of sialic acid?
The substrate is fetuin, a heavily sialidated protein from fetal bovine serum. Our assay is based on the detection of N-acetyl neuraminic acid (NANA, Neu5Ac) in a coupled enzyme reaction leading to the formation of a colored and fluorescent product. Influenza neuraminidase will cleave terminal NANA residues from fetuin.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Lida, M. et al (2019). A sialo-oligosaccharide-rich mucin-like molecule specifically detected in the submandibular glands of aged mice. Archives of oral biology, 97, 52-58. Assay: Neuraminidase in mouse salivary gland. Itoh, Y et al (2015). Emergence of H7N9 Influenza A Virus Resistant to Neuraminidase Inhibitors in Nonhuman Primates. Antimicrob Agents Chemotherapy. 59(8):4962-73. Assay: Neuraminidase in macaque fluid samples. Jihong L, et al (2014). The Sialidases of Clostridium perfringens Type D Strain CN3718 Differ in Their Properties and Sensitivities to Inhibitors. Appl Environ Microbiol. 80(5): 1701-1709. Assay: Neuraminidase in Clostridium perfringens cells. Subathra, M et al (2014). Evaluation of antibody response in mice against avian influenza A (H5N1) strain neuraminidase expressed in yeast Pichia pastoris. J Biosci. 39(3):443-51. Assay: Neuraminidase in Pichia pastoris cells. Subathra, M et al (2014). Evaluation of antibody response in mice against avian influenza A (H5N1) strain neuraminidase expressed in yeast Pichia pastoris. Journal of Biosciences: 1-9. Assay: Neuraminidase in yeast purified Lysate. Li, J., et al. (2013) CodY is a global regulator of virulence-associated properties for. Clostridium perfringens: 00770-13. Assay: Neuraminidase in C. perfringens cells.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
