生物测定系统/QuantiChrom&trade；乳酸脱氢酶细胞毒性检测试剂盒/100试验/C2LD-100
商品编号：
C2LD-100
品牌：
bioassaysys
市场价：
¥1980.00
美元价：
990.00
产品分类：
ELISPOT试剂盒
公司分类：
ELISPOT
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ LDH Cytotoxicity Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.
Key Features
- Safe. Non-radioactive assay (cf. chromium release assay).
- Fast. The whole procedure take 20 min.
- Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day.
Method
- OD500nm
Samples
- Cell culture
Species
- All
Size
- 100 tests
Shelf Life
- 12 months (-20°C)
More Details
- Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme that upon membrane damage is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage. The colorimetric LDH release assay is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.
Does this assay kit work for a particular cell line?
Yes, this kit works for all cell lines of any species.Why is there no standard for this assay?
This assay calculates the percentage of cell death. Therefore it uses the Total Lysis well and the No Treatment Control well (instead of a standard) to calculate the percentage of cell death in your samples.How long do I need to incubate my test compounds with my cells?
This depends entirely on your test compound and your experiment. The incubation can be as short as 5 minutes to as long as one day. The Triton-X 100 total lysis reagent acts immediately to lyse the cells and can be read immediately if so desired.What is the best way to store the reagent?
If you will be using the assay constantly every day, then store in the refridgerator. For long term storage, freeze the reagent at -20°C.When does the assay expire?
The shelf life of the assay is at least one year after receipt at -20°C.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Flage, B. (2018). Heme-induced acute lung injury by inflammasome activation in vascular endothelium (Doctoral dissertation, University of Pittsburgh). Assay: Cytotoxicity in human tissues. To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
