生物测定系统/Enzyfloo&trade；胶原蛋白检测试剂盒/100次检测/ECOL-100
商品编号：
ECOL-100
品牌：
bioassaysys
市场价：
¥7380.00
美元价：
3690.00
产品分类：
免疫组化染色试剂盒
公司分类：
Immunostain_staining_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyFluo™ Collagen Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Collagen determination in biological and cosmetic products.
Key Features
- Safe. Non-radioactive assay.
- Fast and sensitive. Linear detection range (20µL sample): 2 µg/mL to 50µg/mLcollagen in 96-well plate assay.
- Convenient and high-throughput."Add-mix-read" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day.
Method
- FL375/465nm
Samples
- Biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution).
Species
- All
Size
- 100 tests
Detection Limit
- 2 µg/mL
Shelf Life
- 6 months
More Details
- COLLAGEN is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties. Assay methods available for quantifying collagen currently range from needing extensive hydrolysis procedures with acids and bases to using expensive antibodies and complicated protocols. BioAssay Systems collagen assay kit delivers a very simple, safe, and sensitive method to quantify collagen in samples. In the first step of this procedure, collagen in the sample is enzymatically digested into peptides. Subsequently, the N-terminal glycine containing peptides react with the dye reagent to form a fluorescent complex. The fluorescence intensity of this product, measured at λex/em = 375/465 nm, is directly proportional to collagen concentration in the sample.
Does this assay measure hydroxyproline?
No, this assay does not measure hydroxyproline. This assay quantifies N-terminal glycine containing peptides from an enzyme based degradation of collagen.My standard curve isn’t turning out linear / I am getting inconsistent results?
You may also wish to incubate the digest mix step for an extra 30 minutes at 37°C (90 minutes total) to ensure that the digest reaction is complete.I’m not getting the same fluorescence readings for my standards as the standard curve on the protocol?
Fluorescence is measured differently depending on the plate reader. This is why plate readers use relative fluorescent units (RFUs) to quantitate the fluorescence readings. These are arbitrary units to assess the fluorescence intensity of the wells relative to each other. The numbers you see on the standard curve in the protocol are from the assay being performed on our lab’s plate readers. It is completely normal for your assay to produce different fluorescent readings; what is more important is to focus on the linearity of your standard curve.My sample, which I know has collagen, isn’t measuring any collagen with this assay?
Either your samples are diluted too much, or have not been diluted enough. We recommend running a serial dilution on your sample to determine the optimal dilution factor for quantification. This is the range at which dilutions start producing similar collagen concentration estimates after being corrected for dilution factor.Can I use this assay to measure cosmetic or beauty product collagen?
Yes, this assay can be used on store bought collagen. Because the collagen is often very concentrated, you will likely need to dilute it greatly (e.g.100-1000 fold) for it to be within the assay’s detection range.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
