生物测试系统/酶学与贸易；细胞毒性试验试剂盒/100试验/ECTX-100
商品编号：
ECTX-100
品牌：
bioassaysys
市场价：
¥4580.00
美元价：
2290.00
产品分类：
组织标本
公司分类：
Tissue_samples
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyLight™ Cytotoxicity Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents.
Key Features
- Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
- Sensitive and accurate. As low as 50 cells can be quantified.
- Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
- Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- Luminescence
Samples
- Cells
Species
- All
Size
- 100 tests
Detection Limit
- 50 cells
Shelf Life
- 6 months
More Details
- Adenosine 5’-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as ?energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. BioAssay Systems' EnzyLight™ Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells. This non-radioactive, homogeneous cell-based assay can be conveniently performed in microplates. The reagent is compatible with all culture media and liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Massa, S.M., et al. (2010). Small molecule BDNF mimetics activate TrkB signaling and prevent neuronal degeneration in rodents. J Clin Invest 120(5):1774-85. Assay: Cytotoxicity in mouse fibroblast cells.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
