当前位置:
首页
>
产品中心 >
RACE_Reagent_kit >
BioAssay Systems/CellQuanti-MTT™ Cell Viability Assay Kit/500 tests/CQMT-500
BioAssay Systems/CellQuanti-MTT™ Cell Viability Assay Kit/500 tests/CQMT-500
商品编号:
CQMT-500
品牌:
bioassaysys
市场价:
¥3300.00
美元价:
1650.00
产品分类:
RACE试剂盒
公司分类:
RACE_Reagent_kit
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
CellQuanti-MTT™ Cell Viability Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents.
Key Features
- Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
- Sensitive and accurate. As low as 950 cells can be accurately quantified.
- Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.
- Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
- Robust and amenable to HTS. Z factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems.
Method
- OD570nm
Samples
- Cell culture
Species
- All
Size
- 500 tests
Detection Limit
- 950 cells
Shelf Life
- 12 months
More Details
- The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis). The CellQuanti-MTT™ assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 - 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Harbison RA, et al (2011). Calpain plays a central role in 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity in cerebellar granule neurons. Neurotox Res. 19(3):374-88. Assay: Cell viability in rat primary cells. Kalaivani T, et al (2011). Free Radical Scavenging, Cytotoxic and Hemolytic Activities from Leaves of Acacia nilotica (L.) Wild. ex. Delile subsp. indica (Benth.) Brenan. Evid Based Complement Alternat Med. 2011:274741. Assay: Cell viability in monkey Vero cells and Hela cells. Roopan, SM and Khan, FRN (2011). SnO2 nanoparticles mediated nontraditional synthesis of biologically active 9-chloro-6,13-dihydro-7-phenyl-5H-indolo [3,2-c]-acridine derivatives. Med. Chem. Res. 20(6): 732-7. Assay: Cell viability in human erythrocytes.Andre FM, et al (2010). Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death. Bioelectrochemistry 79(1):95-100. Assay: Cell viability in human, mouse cell lines. Chen TS, et al (2010). Delineating biological pathways unique to embryonic stem cell-derived insulin-producing cell lines from their noninsulin-producing progenitor cell lines. Endocrinology 151(8):3600-10. Assay: Cell viability in mouse stem cells. Cheng H, et al (2010). Transient receptor potential melastatin type 7 channel is critical for the survival of bone marrow derived mesenchymal stem cells. Stem Cells Dev. 19(9):1393-403. Assay: Cell viability in mouse stem cells. Khanna, VG et al (2010). Non-Proliferative Activity Of Saponins Isolated From The Leaves Of Gymnema Sylvestre And Eclipta Prostrata On Hepg2 Cells- In Vitro Studyijpsr 1(8): 38-42. Assay: Cell viability in human, monkey HepG2 cells and Vero cells.Poh CK, et al (2010). The effect of VEGF functionalization of titanium on endothelial cells in vitro. Biomaterials 31(7):1578-85. Assay: Cell viability in human endothelial cell. Yoshida H, et al (2010). The citrus flavonoids hesperetin and naringenin block the lipolytic actions of TNF-alpha in mouse adipocytes. Biochem Biophys Res Commun 394(3):728-32. Assay: Cell viability in mouse adipocytes. Gupta V et al (2009). Fabrication and characterization of silk fibroin-derived curcumin nanoparticles for cancer therapy. Int. J. Nanomedicine 4: 115-122. Assay: Cell viability in human cells. Haidar ZS,et al (2009). Modulated release of OP-1 and enhanced preosteoblast differentiation using a core-shell nanoparticulate system. J Biomed Mater Res A. 91(3):919-28. Assay: Cell viability in mouse preosteoblast cells. Khanna VG, Kannabiran K (2009). Anticancer-cytotoxic activity of saponins isolated from the leaves of Gymnema sylvestre and Eclipta prostrata on HeLa cells. Int J Green Pharm.3:227-9. Assay: Cell viability in human Hela Cells.Roopan, SM and Nawaz, FR (2009). Synthesis, antioxidant, hemolytic and cytotoxicity activity of AB ring core of mappicine. ARKIVOC 2009 (xiii) 161-169. Assay: Cell viability in human, monkey cell lines.Suthindhiran, K and Kannabiran, K (2009). Cytotoxic and Antimicrobial Potential of Actinomycete Species Saccharopolyspora salina VITSDK4 Isolated from the Bay of Bengal Coast of India. Am. J. Infect. Diseases 5 (2): 90-98. Assay: Cell viability in mouse Hela Cells.Zhang Y,et al (2009). Estrogen inhibits glucocorticoid action via protein phosphatase 5 (PP5)-mediated glucocorticoid receptor dephosphorylation. J Biol Chem.284(36):24542-52. Assay: Cell viability in human MCF-7 cells. Desplats PA, et al (2008). Functional roles for the striatal-enriched transcription factor, Bcl11b, in the control of striatal gene expression and transcriptional dysregulation in Huntington"s disease. Neurobiol Dis. 31(3):298-308. Assay: Cell viability in human cells. Du Y, et al (2008). Oridonin confers protection against arsenic-induced toxicity through activation of the Nrf2-mediated defensive response. Environ Health Perspect. 116(9):1154-61. Assay: Cell viability in human cell lines. Nelson GN et al (2008). Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissueengineered vascular grafts in vivo. FASEB J 22 (11): 3888-95. Assay: Cell viability in human cells. Padmini, E et al (2008). Differential HSP90 expression in fish hepatocytes from polluted estuary during summer. Fisheries Science 74(5): 1118-26. Assay: Cell viability in fish hepatocyte.Yonei N, et al (2007). Induction of PDGF-B in TCA-treated epidermal keratinocytes. Arch Dermatol Res. 299(9):433-40. Assay: Cell viability in mouse keratinocytes and fibroblasts. Oxelmark E, et al (2006). The cochaperone p23 differentially regulates estrogen receptor target genes and promotes tumor cell adhesion and invasion. Mol Cell Biol. 26(14):5205-13. Assay: Cell viability in human cell lines.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部,是一家专注于提供高质量试剂盒的生物技术公司,试剂盒种类广泛、使用简便、操作简单、快速且性能优越,用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量(可用于HTS药物筛选)、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征,且采用发光、生物发光、化学发光、荧光等非放射性的检测技术,安全无害可靠。