生物测定系统/酶染色与贸易；D-氨基酸检测试剂盒/100次检测/EDAA-100
商品编号：
EDAA-100
品牌：
bioassaysys
市场价：
¥8380.00
美元价：
4190.00
产品分类：
蛋白交联剂
公司分类：
Crosslinkers
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ D-Amino Acid Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Quantitative determination of D-Amino Acids in biological and other samples.
Key Features
- Fast and sensitive. Linear detection range: 0.86 to 500 µM (colorimetric assay) and 0.18 to 50 µM (fluorimetric assay) for 60 min reaction.
- Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day.
Method
- OD570nm or FL530/585nm
Samples
- Tissue, milk, and other biological samples.
Species
- All
Size
- 100 tests
Detection Limit
- 0.86 μM (colorimetric assay), 0.18 μM (fluorimetric assay)
Shelf Life
- 6 months
More Details
- D-AMINO-ACIDS are not as widespread as their enantiomeric counterparts in proteins but they can be found in organisms ranging from bacteria (cell walls and antibiotics) to mammals (central nervous systems). The presence of D-amino acids in food is also of considerable interest. Racemization of L-amino acids during food processing may affect food quality and nutritional value.BioAssay Systems' D-amino acid assay uses an enzyme-catalyzed oxidation of D-amino acids to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the D-amino acid concentration in the sample.
Will this kit detect L-amino acids?
The DAA enzyme is specific to D-amino acids only.What samples have you tested?
This kit has been tested on rat brain tissue, mouse brain tissue, cerebrospinal fluid, and dairy products like whole milk.Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol.How do you use this kit on cerebrospinal fluid?
CSF can be assayed directly and an internal standard should be used.Samples will need three separate reactions: 1) sample plus standard 2) sample alone 3) sample blank. For the internal standard prepare 200 µL 1250 µM standard by mixing 125 µL 2 mM Standard and 75 µL dH2O. For the sample plus standard well, add 5 µL 1250 µM standard and 20 µL sample. For the sample and sample blank wells, add 5 µL dH2O and 20 µL sample. Prepare enough Working Reagent (WR) for all assay wells by mixing, for each well, 85 µL Assay Buffer, 1 µL DAA Enzyme, 1 µL HRP Enzyme, 1 µL Dye Reagent. Prepare a WR without the DAA Enzyme for the sample blank well(s). Add 80 µL of the WR to each well. Tap plate briefly to mix. Incubate at room temperature for 60 min. Use a plate reader to read OD570nm. The sample D-amino acid concentration is computed as follows:
where RSAMPLE, RBLANK, and RSTANDARD are the OD or fluorescence values of the Sample, Sample Blank, and the Sample plus Standard respectively. n is the sample dilution factor. Note: The volume of the internal standard is 4× lower than the sample volume; thus, the internal standard concentration should be divided by 4.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
