生物测定系统/酶染色与贸易；肌酸测定试剂盒/100试验/ECRT-100
商品编号：
ECRT-100
品牌：
bioassaysys
市场价：
¥7180.00
美元价：
3590.00
产品分类：
多聚物
公司分类：
polymerizer
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Creatine Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.
Key Features
- High sensitivity and wide linear range. Use 10 μL sample. Linear detection range 4 to 1000 μM (colorimetric) or 0.5 to 50 μM (fluorimetric).
- Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes.
Method
- OD570nm, or FL530/585nm
Samples
- Serum, plasma, urine, saliva etc
Species
- All
Size
- 100 tests
Detection Limit
- 4 μM
Shelf Life
- 12 months
More Details
- CREATINE is present in vertebrates and helps to supply energy to muscle. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases. Simple, direct and automation-ready procedures for measuring creatine are popular in research and drug discovery. BioAssay Systems creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to the creatine concentration in the sample.
Can this kit be used to measure creatine concentrations in cells?
Yes. To determine intracellular creatine concentrations, harvest cells (0.1-2 million cells per assay) in a 1.5mL tube, centrifuge 1-2 min at 3,000 rpm on a table centrifuge. Remove culture medium, wash cells quickly with cold PBS. Immediately remove any PBS. Prepare Working Reagent. Add 120 µL WR directly to the cell pellet and 80 µL WR to standards in 96-wells. Vortex cell sample tube 1 min, incubate 30 min at room temp. Centrifuge 5 min at 14,000 rpm. Transfer 100 µL supernatant to 96-well. Read OD570nm on a plate reader. Notes: 1. cell number. It is prudent to run several doses of cells, e.g. 0.1, 1, 2×106 cells to determine optimal cell number to be used in subsequent assays. Working Reagent should contain a lysis reagent. BioAssay Systems will supply this reagent upon request.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Wang, Yujie, et al (2018). Guanidinoacetic Acid Regulates Myogenic Differentiation and Muscle Growth Through miR-133a-3p and miR-1a-3p Co-mediated Akt/mTOR/S6K Signaling Pathway. International journal of molecular sciences 19.9: 2837. Assay: Creatine in murine cells. Chan, Yiumo Michael, et al (2017). Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model. PloS one 12.3: e0173261. Assay: Creatine in human and mouse tissues. Holz, Josefin-Beate, and Alex Hemeryck (2017). Inhibition of bone resorption with rankl binding peptides. U.S. Patent Application No. 15/295,229. Assay: Creatine in human urine.Schoborg, JA et al (2014). Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnology Journal 9(5): 630-640. Assay: Creatine in yeast cells. Qi S et al (2012). Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics. Hepatol Res 42(7):677-85. Assay: Creatine in human serum.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
