生物测定系统/酶染色与贸易；胆碱分析试剂盒/100试验/ECHO-100
商品编号：
ECHO-100
品牌：
bioassaysys
市场价：
¥7380.00
美元价：
3690.00
产品分类：
WB试剂盒
公司分类：
Westornblot
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Choline Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of choline and evaluation of drug effects on choline metabolism.
Key Features
- Use 20 μL samples. Linear detection range: colorimetric assay 1 to 100 μM, fluorimetric assay 0.2 to 10 μM choline.
Method
- OD570nm, or FL530/585nm
Samples
- Serum, plasma, urine, saliva, milk, tissue, cells etc
Species
- All
Size
- 100 tests
Detection Limit
- OD, FL: 1, 0.2 μM
Shelf Life
- 6 months
More Details
- CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
What are known interferences?
Chondroitin sulfate C (chondroitin-6-sulfate) appears to interfere with the assay; creating false positive results.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Ballester-Lozano, GF et al (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma. Benedito-Palos, L et al (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Choline in fish plasma.Ballester-Lozano, GF et al (2014). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma.Strilakou, AA et al (2014). Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats. Canadian Journal of Physiology and Pharmacology 92(1): 78-84. Assay: Choline in rat serum.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
