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生物测定系统/QuantiChrom&trade；生物素分析试剂盒/100次试验（96孔）、320次试验（384孔）/DBIO-100
生物测定系统/QuantiChrom&trade；生物素分析试剂盒/100次试验（96孔）、320次试验（384孔）/DBIO-100
商品编号：
DBIO-100
品牌：
bioassaysys
市场价：
¥3900.00
美元价：
1950.00
产品分类：
纯水机
公司分类：
water_purifier
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Biotin Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Quantitative determination of biotin in food, cosmetic, and biotinylated proteins.
Key Features
- Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well).
- Convenient. The procedure involves adding a single working reagent and reading after 20 minutes.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.
Method
- OD500nm
Samples
- Food, cosmetics, supplements, and biotinylated proteins or antibodies
Species
- All
Size
- 100 tests (96-well), 320 tests (384-well)
Detection Limit
- 8 μM
Shelf Life
- 12 months
More Details
- BIOTIN , or Vitamin B7, is a water soluble vitamin involved in metabolism, cell growth, and protein synthesis. It is a cofactor to multiple carboxylases necessary for metabolizing fatty acids, glucose, and amino acids. Biotin is found in a wide range of foods and is often taken as a dietary supplement. In the biotechnology industry, biotin is commonly conjugated to proteins in a process called biotinylation. These biotinylated proteins can then be specifically selected using streptavidin and/or avidin's strong affinity for biotin in biochemical assays such as ELISAs.BioAssay Systems' Biotin Assay Kit is based on avidin's weak affinity for 4'-hydroxyazobenzene-2-carboxylic acid (HABA) and strong affinity for biotin. The avidin-HABA complex reagent has an absorbance at 500nm. When the colored avidin-HABA reagent is introduced to biotin, the biotin binds to avidin, displacing the HABA and causing a decrease in absorbance. The decrease in absorbance at 500nm is directly proportional to the biotin in the sample.
The blank value is higher than the standard values. What is wrong?
This is completely normal. The assay is measuring the decrease in absorbance at 500nm. The higher the concentration of biotin, the lower the absorbance will be in the calculation.My sample is reporting much lower levels of biotin than I was expecting?
The biotin levels in your sample may be significantly greater than the kit detection limit. We recommend diluting your sample and assaying again. You may wish to perform a serial dilution on the sample to determine the optimal dilution factor. Aim for a dilution that yields a concentration read out somewhere in the middle of the standard curve. Correct for the dilution factor in the calculation.Some of my samples are getting negative concentration read outs?
This is likely indicative no biotin or biotin below the kit detection limit.The kit arrived thawed and at room temperature, but the protocol says to store it at -20°C. Is the kit still ok to use?
Yes, the kit is shipped at room temperature and is stable for the duration of shipment at that temperature. Upon receipt, the kit should be stored at -20°C.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
