生物测定系统/QuantiFluo&trade；二胺氧化酶检测试剂盒/100试验/QFDO-100
商品编号：
QFDO-100
品牌：
bioassaysys
市场价：
¥8580.00
美元价：
4290.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiFluo™ Diamine Oxidase Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of diamine oxidase in biological samples (e.g. serum, plasma etc)
Key Features
- Fast and sensitive. Use of 10 μL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
- FL530/585nm
Samples
- Serum, Plasma
Species
- All
Size
- 100 tests
Detection Limit
- 0.5 U/L
Shelf Life
- 6 months
More Details
- DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT. BioAssay Systems non-radioactive, fluorimetric DAO assay is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λex/em = 530/585 nm is directly proportional to the enzyme activity. • Enzyme-Activity-Assays.htm" target="_blank">Other Enzyme Activity Assay Kits • Metabolism.htm" target="_blank">Other Metabolism Assay Kits
How do I store the kit?
This kit is shipped at room temperature. Upon receiving, please keep the kit in the freezer (-20 to -10°C) for long term stability.What samples have you tested?
Serum samples were tested. Since DAO is copper dependent, EDTA should be avoided in sample preparation.What is the benefit of using an internal standard?
Serum and plasma samples greatly influence the HRP reaction. In order to measure an appropriate ?RFU for H2O2 in a serum or plasma matrix, an internal standard is required.Will the assay kit work in a 384 well plate, cuvette, etc?
Yes, the assay can be used in any standard black plate or cuvette. Simply adjust the total reaction volume to your container. For 384 well use 20-90 μL, for a cuvette use 1 mL (some narrow cuvettes can hold 200-500 μL).Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 1 hour.I don’t have the correct wavelength filter, will the assay still work?
Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
