生物测定系统/酶染色与贸易；富马酸盐检测试剂盒/100次检测/EFUM-100
商品编号：
EFUM-100
品牌：
bioassaysys
市场价：
¥6980.00
美元价：
3490.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Fumarate Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of fumarate and drug effects on its metabolism.
Key Features
- Fast and sensitive. Use of 20 µL sample. Linear detection range 0.005 to 2 mM fumarate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density after 30 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
- OD565nm
Samples
- Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum)
Species
- All
Size
- 100 tests
Detection Limit
- 5 µM
Shelf Life
- 6 months
More Details
- FUMARATE , or Fumaric Acid, is one of the key components in TCA cycle and is used by cells to form ATP. Human skin when exposed to sunlight will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industries. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs cycle function, a defect in the enzyme fumarase or mitochondrial function. BioAssay Systems fumarate assay kit is based on fumarase catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm is proportional to the fumarate concentration in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
