生物测定系统/酶染色与贸易；乌头酸酶检测试剂盒/100次检测/EACO-100
商品编号：
EACO-100
品牌：
bioassaysys
市场价：
¥6380.00
美元价：
3190.00
产品分类：
ELISPOT试剂盒
公司分类：
ELISPOT
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Aconitase Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
Key Features
- Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 100 U/L for 20 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day.
Method
- OD565nm
Samples
- Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species
- All
Size
- 100 tests
Detection Limit
- 0.5 U/L
Shelf Life
- 6 months
More Details
- ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich’s ataxia and diabetes. BioAssay Systems' aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Stein, C. S., Jadiya, P., Zhang, X., McLendon, J. M., Abouassaly, G. M., Witmer, N. H. & Boudreau, R. L. (2018). Mitoregulin: A lncRNA-encoded microprotein that supports mitochondrial supercomplexes and respiratory efficiency. Cell reports, 23(13), 3710-3720. Assay: Aconitase in human tissue.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
