生物测定系统/酶染色与贸易；草酸盐检测试剂盒/100次检测/EOXA-100
商品编号：
EOXA-100
品牌：
bioassaysys
市场价：
¥9780.00
美元价：
4890.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Oxalate Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of oxalate (oxalic acid) in urine and other samples.
Key Features
- Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 10 minute incubation: 20 to 1500 µM oxalate.
- Fast and convenient. Sample pre-treatment is faster and easier than using activated carbon in competitor’s assay kits.
- High-throughput adaptable. The procedure involves addition of a single working reagent and incubation for 10 min at room temperature. Can be automated for processing thousands of samples per day.
Method
- OD595nm
Samples
- urine, animal and plant tissue samples.
Species
- All
Size
- 100 tests
Detection Limit
- 20 µM
Shelf Life
- 6 months
More Details
- Oxalate or Oxalic Acid is a metabolic breakdown product of the Kreb's Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine. Simple and high-throughput assays for measuring oxalate concentration find wide applications. BioAssay Systems' oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.
How do I store the kit?
This kit is shipped on ice. Upon receiving, please keep the kit in the freezer (-20 to -10°C).What samples have you tested?
The assay kit has been tested with human serum, rat serum, human plasma, plant tissue samples, and human urine. Please follow the directions on the protocol for sample pretreatment.How do I prepare cell or tissue samples for assays?
Follow the general protocol below for cell and tissue samples.Cell Samples. Suspend about two million (2 × 106) harvested cells in 400 µL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in a ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.Tissue Samples. Cut the tissue into small pieces and weigh 20-100 mg tissue, then add 200- 1000 µL ice-cold PBS. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice water bath). The degree of tissue lysis can be checked under a microscope. Centrifuge homogenate at 14,000g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.What is the benefit of using an internal standard?
Some assays encounter interference and the values cannot be compared to a standard curve. The use of an internal standard corrects for potential interference.I don’t have the correct wavelength filter, what other wavelength(s) would work?
The assay can be measured at 550 - 600 nm with 595 nm as the peak. Reading outside this range will result in a significant decrease in sensitivity.Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 2 hours.Do I need to use a standard or standard curve with each assay run?
Yes, it is highly recommended.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Fukano, Y. (2017). Vine tendrils use contact chemoreception to avoid conspecific leaves. Proceedings of the Royal Society B: Biological Sciences, 284(1850), 20162650. Assay: Oxalate in leaf tissue.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
