生物测定系统/酶染色与贸易；谷氨酸测定试剂盒/100试验/EGLT-100
商品编号：
EGLT-100
品牌：
bioassaysys
市场价：
¥7180.00
美元价：
3590.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Glutamate Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism.
Key Features
- Sensitive and accurate. Detection limit of 50 μM, linearity up to 2.5 mM glutamate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
- OD565nm
Samples
- Serum, plasma, urine, cell, tissue etc
Species
- All
Size
- 100 tests
Detection Limit
- 50 μM
Shelf Life
- 6 months
More Details
- Glutamateis an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable. BioAssay Systems EnzyChrom™ glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Kansakar, U. (2019). Development of an Astrocyte/Glioma Co-Culture System for Measuring Cellular Dynamics. Assay: Glutamate in rat cells.Izrael, Michal, et al (2018). Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1 G93A and NSG animal models. Stem cell research & therapy 9.1: 152. Assay: Glutamate in human cells. Das, Champak, et al (2017). Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe. Sensors and Actuators B: Chemical 238: 633-640. Assay: Glutamate in glucose. Swiercz, Rafal, et al (2017). Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget 8.2: 3528-3541. Assay: Glutamate in human cells. Izrael, Michal, et al (2016). Directed differentiation of astrocytes from human pluripotent stem cells for use in drug screening and the treatment of amyotrophic laterial sclerosis (als). U.S. Patent Application No. 15/025,946. Assay: Glutamate in human cells.Kahlert, Ulf D., et al (2016). Alterations in cellular metabolome after pharmacological inhibition of N otch in glioblastoma cells. International journal of cancer 138.5: 1246-1255. Assay: Glutamate in human cells. Wray, Robin, et a (2016). Dihydrostreptomycin directly binds to, modulates, and passes through the MscL channel pore. PLoS biology 14.6: e1002473. Assay: Glutamate in E. coli cells. Mesci, P et al (2015). System xC- is a mediator of microglial function and its deletion slows symptoms in amyotrophic lateral sclerosis mice. Brain. 138(Pt 1):53-68. Assay: Glutamate in mice spinal cord tissue. Iscla, I et al (2014). Streptomycin potency is dependent on MscL channel expression. Nature Communications. 5:4891. Assay: Glutamate in E. coli vector. Ramos, C et al (2012). Native Presynaptic Metabotropic Glutamate Receptor 4 (mGluR4) Interacts with Exocytosis Proteins in rat Cerebellum. Journal of Biological Chemistry 287(24):20176-86. Assay: Glutamate in rat protein lysate. Liang, Y., et al. (2011). Additive effect of tetramethylpyrazine and deferoxamine in the treatment of spinal cord injury caused by aortic cross-clamping in rats. Spinal Cord 49(2):302-6. Assay: Glutamate in rat. Rink, C., et al (2011). Oxygen-inducible glutamate oxaloacetate transaminase as protective switch transforming neurotoxic glutamate to metabolic fuel during acute ischemic stroke. Antioxid Redox Signal 14(10):1777-85. Assay: Glutamate in rat brain tissue. Yang, LM and Blount, P. (2011). Manipulating the permeation of charged compounds through the MscL nanovalve. FASEB J 25(1):428-34. Assay: Glutamate in E. coli.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
