生物测定系统/酶染色与贸易；甲酸盐检测试剂盒/100次检测/EFOR-100
商品编号：
EFOR-100
品牌：
bioassaysys
市场价：
¥8380.00
美元价：
4190.00
产品分类：
反应底物
公司分类：
Reaction_substrate
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Formate Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Direct determination of formate concentrations in biological (e.g. urine, blood, etc.) and other samples.
Key Features
- Fast and sensitive. Use of 10 µL sample. Linear detection range 0.050 to 5 mM Formate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
- OD565nm
Samples
- Urine, Serum etc
Species
- All
Size
- 100 tests
Detection Limit
- 50 μM
Shelf Life
- 6 months
More Details
- FORMATE(HCOO-) is the anion derived from formic acid, the simplest carboxylic acid. It is also the metabolic byproduct of formaldehyde metabolism in our body, and the eventual metabolic byproduct of methanol which is first broken down to formaldehyde. At high levels, formate is neurotoxic to the central nervous system and can cause blindness, coma, and death. Although naturally present in the body at low levels, elevated levels of formate may be used as an indicator of formaldehyde exposure and methanol poisoning. BioAssay Systems formate assay kit is based on formate dehydrogenase catalyzed oxidation of formate, which generates carbon dioxide and NADH that reduces a formazan (MTT) dye. The intensity of the reduced MTT, measured at 565 nm, is directly proportional to formate concentration in the sample.
How do I store the kit?
The Kit is shipped on ice. Upon receiving, please keep the kit in the freezer (-20 to -10°C)Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided, especially with enzymes; aliquot enzyme for storage to avoid unnecessary freeze/thaw cycles. Working reagent should be made fresh each day.What samples have you tested?
We have tested the kit thoroughly on human urine. We have also tested the kit on human serum and bovine serum. The assay has been tested on typical concentrations of EDTA, Heparin, and Citrate used for anticoagulation in plasma and they did not show any significant interferences.Do I need to use a standard or standard curve with each assay run?
Yes, it is highly recommendedWill the assay kit work in a 384 well plate, cuvette, etc?
Yes, the assay can be used in any standard plate and cuvette. Simply adjust the total reaction volume to your container. For 384 well use 20-90 μL, for a cuvette use 1 mL (some narrow cuvettes can hold 200-500 μL).Does the assay work with saliva samples?
Yes, the assay has been tested and works with porcine saliva samples. Other saliva samples should work similarly.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
