生物测定系统/QuantiChrom&trade；铜分析试剂盒/250测试/DICU-250
商品编号：
DICU-250
品牌：
bioassaysys
市场价：
¥8380.00
美元价：
4190.00
产品分类：
细胞研究
公司分类：
Cell_Research
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Copper Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism.
Key Features
- Sensitive and accurate. Linear detection range 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM) copper in 96-well plate assay.
- Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
- Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Method
- OD359nm
Samples
- Biological, environment, food and beverage
Species
- All
Size
- 250 tests
Detection Limit
- 7 μg/dL (1.0 μM)
Shelf Life
- 12 months
More Details
- Copperis an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper is mainly present in caeruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anaemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson’s disease, microcytic hypochromic anaemia and bone disease due to reduced collagen synthesis. Simple, direct and automation-ready procedures for measuring copper concentrations find wide applications in research, drug discovery and environmental monitoring. BioAssay Systems copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured at 359nm, is directly proportional to copper concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Parmar, A., Pascali, G., Lerra, L., Yee, E., Ahmed-Cox, A., Kimpton, K. & Liu, G. J. (2018). In vivo [64Cu] CuCl2 PET imaging reveals activity of Dextran-Catechin on tumor copper homeostasis. Theranostics, 8(20), 5645-5659. Assay: Copper in neuroblastoma cell. Dikicioglu, D., & Oliver, S. G. (2017). Estimating global enzyme abundance levels from cofactor requirements: a model-based analysis of the iron metabolism in yeast. bioRxiv, 229104. Assay: Copper in S. cerevisiae cells.Yee, E. M., Brandl, M. B., Pasquier, E., Cirillo, G., Kimpton, K., Kavallaris, M. & Vittorio, O. (2017). Dextran-Catechin inhibits angiogenesis by disrupting copper homeostasis in endothelial cells. Scientific reports, 7(1), 7638. Assay: Copper in human cells.Vittorio, O., Brandl, M., Cirillo, G., Kimpton, K., Hinde, E., Gaus, K. & Haber, M. (2016). Dextran-Catechin: An anticancer chemically-modified natural compound targeting copper that attenuates neuroblastoma growth. Oncotarget 7(30): 47479-47493. Assay: Copper in human cells. EL-Deeb, W. M., & El-Bahr, S. M. (2014). Selected Biochemical Indicators of Equine Rhabdomyolysis in Arabian Horses: Acute Phase Proteins and Trace Elements. Journal of Equine Veterinary Science 34(4): 484-488. Assay: Copper in horse serum. Bartnikas TB (2012) Known and potential roles of transferrin in iron biology. Biometals 25(4):677-86. Assay: Copper in human protein. Philips N et al (2012) Beneficial regulation of fibrillar collagens, heat shock protein-47, elastin fiber components, transforming growth factor-beta1, vascular endothelial growth factor and oxidative stress effects by copper in dermal fibroblasts. Connect Tissue Res. 53(5):373-8. Assay: Copper in human cell. Piret JP et al (2012) Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape. Nanotoxicology 6:789-803. Assay: Copper in human cell. Piret, JP et al (2012). Copper (II) oxide nanoparticles penetrate into HepG2 cells, exert cytotoxicity via oxidative stress and induce pro-inflammatory response. Nanoscale 4(22): 7168-7184. Assay: Copper in human cell culture medium. Lull ME, et al (2008). Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass. Pediatr Res. 63(6):638-44. Assay: Copper in human plasma.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
