生物测定系统/QuantiChrom&trade；铬分析试剂盒/250试验/DCRM-250
商品编号：
DCRM-250
品牌：
bioassaysys
市场价：
¥7980.00
美元价：
3990.00
产品分类：
ELISA试剂盒
公司分类：
ELISA_Reagent_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Chromium Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of chromium Cr(VI).
Key Features
- Sensitive and accurate. Linear detection range of 20 - 2000 μg/L Chromium.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day.
Method
- OD480nm
Samples
- Biological (serum, plasma etc), environmental (water, soil etc), food and beverage samples
Species
- All
Size
- 250 tests
Detection Limit
- 20 μg/L (0.38 μM, 0.02 ppm)
Shelf Life
- 6 months
More Details
- CHROMIUM is widely used in various industries such as electroplating, leather tanning, chrome paint, dying, hardened steel, ceramic and glass industry. Chromium exists in two stable oxidation states, hexavalent Cr(VI) and trivalent Cr(III). Cr(VI) is produced solely by industrial processes, whereas in nature, chromium exists in its trivalent form. Cr(III) is generally regarded as nontoxic due to poor absorption. Cr(VI) is considered a pulmonary carcinogen and has tested positive in genotoxicity tests. It is one of the most serious pollutants in many water streams due to its carcinogenic potential. Most countries apply a legal limit of 50-100 μg/L Cr in drinking water. BioAssay Systems Chromium Assay Kit provides a simple one-step colorimetric means to directly measure Cr(VI) in a sample. In the assay, Cr(VI) forms a stable complex with a specific chromogenic dye. The optical density at 480nm is directly proportionate to the Cr(VI) concentration in the sample. Cr(III) can be converted to Cr(VI) with nitric acid/hydrochloric acid, thus allowing the determination of Cr(III) or total Cr [Cr(III) + Cr(VI)] in the sample. The assay is sensitive with a detection limit of 20 μg/L Cr.
We would like to measure chromium which is incorporated into blood as Cr-EDTA. Can we use this kit for such an application? Does EDTA interfere with this assay?
We have not explicitly tested the chromium assay kit for EDTA interference, but EDTA is likely to interfere with the formation of the colored chromium complex. However, the general sample treatment procedure (see data sheet) may destroy EDTA and release chromium, making it possible to use the assay for this purpose, but we have not tested that.Could you give me more information about the application and sample preparation of the Cr (VI) kit in the analysis of food samples?
Our Chromium Assay Kit directly measures Cr(VI). Cr(VI) is fully soluble and can be easily extracted into water. Therefore, you only need to add water to your food sample, mix well, centrifuge (14,000 g for 10 min), or filtrate through a Whatman No. 42 paper and use the clear supernatant or filtrate as sample for assay. Since the Cr(VI) concentration in the sample is unknown, it is best to use small amount of water so as to achieve a relatively concentrated extract. If the Cr(VI) concentration in the extract is too high, one can dilute it in water and rerun the assay. If you need to determine Cr(III) or total chromium in your sample, you would need to use the general procedure to convert Cr(III) to Cr(VI).For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Su, X., Kushima, A., Halliday, C., Zhou, J., Li, J., & Hatton, T. A. (2018). Electrochemically-mediated selective capture of heavy metal chromium and arsenic oxyanions from water. Nature communications, 9(1), 4701. Assay: Chromium in Environment.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
