生物测定系统/酶染色与贸易；天冬氨酸转氨酶检测试剂盒/100次检测/EASTR-100
商品编号：
EASTR-100
品牌：
bioassaysys
市场价：
¥6380.00
美元价：
3190.00
产品分类：
血清纯化蛋白
公司分类：
Serum_purified_protein
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Aspartate Transaminase Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.
Key Features
- Sensitive. Linear detection range: 2-100 U/L.
- Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.
Method
- OD340nm
Samples
- Serum, plasma etc
Species
- All
Size
- 100 tests
Detection Limit
- 2 U/L
Shelf Life
- 6 months
More Details
- Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma. Simple, direct and automation-ready procedures for measuring AST activity find wide applications in research and drug discovery. BioAssay Systems AST activity assay is based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.
I am not quite clear about the factor 388 used in the calculation. How is path length correction for 220 µl of sample volume in the 96 well done? Is the concentration of the NADH used 10 mM?
The concentration of the NADH solution is 10 mM. The concentration of NADH in the final reaction volume (V = 220 μL) is calculated by accounting for the dilution steps when preparing the working reagent, i.e. 4 μL/206μL in the first step and 200 mL/220 mL in the second step. This is the final initial concentration of NADH in the reaction.To calculate the activity of the sample, i.e. µmoles NADH consumed / (L x min), one has to divide by 5 min incubation time and multiply by the dilution factor of the sample which is 11.Multiplying all these constant values together equals 388.There is no need for a path length correction, because the sample and the standard wells contain the same volume.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Li, Yajuan, et al (2019). PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease. The Journal of clinical investigation 129.3. Assay: Aspartate transaminase in mice serum. Hay, Charles E., et al (2018). Development and testing of AAV-delivered single-chain variable fragments for the treatment of methamphetamine abuse. PloS one 13.6: e0200060. Assay: Aspartate transaminase in mice serum. Kamerkar, Sushrut, et al (2017). Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature 546.7659: 498. Assay: Aspartate transaminase in mice plasma. Kim, Soo Hyun, et al (2017). Ezetimibe ameliorates steatohepatitis via AMP activated protein kinase-TFEB-mediated activation of autophagy and NLRP3 inflammasome inhibition. Autophagy 13.10: 1767-1781. Assay: Aspartate transaminase in mice serum. Sharabi, K et al (2017). Selective chemical inhibition of PGC-1alpha gluconeogenic activity ameliorates type 2 diabetes. Cell 169.1: 148-160. Assay: Aspartate transaminase in mice serum. Wardah, J. Rahmahani, and T. Sopandi (2017). Effect of Phyllanthus buxifolius Leaf as a Feed Supplement on Liver Function and Haematological Response of Quail (Coturnix coturnix japonica) Challenged with Infectious Newcastle Disease Virus. International Journal of Poultry Science 16: 354-363. Assay: Aspartate transaminase in quail serum.Weis, Sebastian, et al (2017). Metabolic adaptation establishes disease tolerance to sepsis. Cell 169.7: 1263-1275. Assay: Aspartate transaminase in mice plasma. Seemann, Semjon, and Amelie Lupp (2016). Administration of AMD3100 in endotoxemia is associated with pro-inflammatory, pro-oxidative, and pro-apoptotic effects in vivo. Journal of biomedical science 23.1: 68. Assay: Aspartate transaminase in mouse serum. Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: aspartate aminotransferase enzyme activity in fish. Laura, BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Aspartate transaminase in fish plasma.Seemann, S and A Lupp (2015). Administration of a CXCL12 Analog in Endotoxemia Is Associated with Anti-Inflammatory, Anti-Oxidative and Cytoprotective Effects In Vivo. PLoS One 10(9): e0138389. Assay: Aspartate transaminase in mouse serum. Jiang, S., et al (2014). Effect of perches on liver health of hens. Poultry science, 93(7), 1618-1622. Assay: Aspartate transaminase in hen serum. Vives, V et al (2014). Pharmacological inhibition of Dock5 prevents osteolysis by affecting osteoclast podosome organization while preserving bone formation. Nature Communications 6: 6128. Assay: Aspartate transaminase in mouse serum.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
