生物测定系统/酶染色与贸易；乙酰胆碱检测试剂盒/100次检测/EACL-100
商品编号：
EACL-100
品牌：
bioassaysys
市场价：
¥7380.00
美元价：
3690.00
产品分类：
封闭血清
公司分类：
Closed_serum
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Acetylcholine Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.
Key Features
- Use 20 μL samples. Linear detection range: colorimetric assay 10 to 200 μM, fluorimetric assay 0.4 to 10 μM acetylcholine.
Method
- OD570nm, or FL530/585nm
Samples
- Serum, plasma, urine, saliva, milk, tissue, and cell culture etc
Species
- All
Size
- 100 tests
Detection Limit
- OD, FL: 10, 0.4 μM
Shelf Life
- 6 months
More Details
- ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
Can the acetylcholine level in human serum be measured with this kit?
We have not tested this assay with blood samples. In the literature search I found the following reference:Plasma levels in young women: 456.1 +/- 53.1 (SEM) pg/mL which equals 3.1 nM. (Kawashima et al. Neuroscience Letters 80:(3), 339-342; 1987.) If this is indeed a representative study, it would mean that our assay is not sensitive enough to detect acetylcholine in plasma or serum samples. Furthermore, plasma levels of choline are much higher (~10 µM) and will thus have to be removed before assaying.What is the principle of this assay?
The assay is based on the enzymatic degradation of acetylcholine to choline and acetate and the subsequent detection of choline.Can this assay measure acetylcholine in brain tissue?
We have not tested acetylcholine assay specifically with brain tissue samples, but believe it should work. Typical concentrations of choline and acetylcholine in brain tissue are 10-70 nmoles/g. So if the tissue sample is suspended in ice-cold PBS at 500 mg/mL, we should expect 5-35 ?M choline or acetylcholine in the supernatant.Suggested protocol: weigh brain tissue (approx. 50 mg) and add 100 µL ice-cold PBS, lyze tissue by homogenization or sonication. Centrifuge at 14,000 rpm for 10 min at 4°C. Carefully transfer the supernatant to a clean tube and perform the assays for choline and acetylcholine.Due to the low concentration range, the fluorescence assay procedure is recommended.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Ahmad, A., Ramasamy, K., Jaafar, S. M., Majeed, A. B. A., & Mani, V. (2014). Total isoflavones from soybean and< i=""> tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain. Food and Chemical Toxicology 65: 120-128. Assay: Acetylcholine in rat brain. Baitharu, I., Jain, V., Deep, S. N., Hota, K. B., Hota, S. K., Prasad, D., & Ilavazhagan, G. (2013). Withania somnifera root extract ameliorates hypobaric hypoxia induced memory impairment in rats. Journal of ethnopharmacology 145(2): 431-441. Assay: Acetylcholine in rat hippocampus. Chen L et al (2012). Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae. Environ Toxicol Chem. 31(10):2338-44. Assay: Acetylcholine in fish. He C, et al (2012). Embryonic exposure to benzo(a)pyrene influences neural development and function in rockfish (Sebastiscus marmoratus). Neurotoxicology 33(4):758-62. Assay: Acetylcholine in fish. He C, et al (2012). Exposure of Sebastiscus marmoratus embryos to pyrene results in neurodevelopmental defects and disturbs related mechanisms. Aquat Toxicol 15(116-117):109-15. Assay: Acetylcholine in fish. Mani V, et al (2012). Effects of the Total Alkaloidal Extract of Murraya koenigii Leaf on Oxidative Stress and Cholinergic Transmission in Aged Mice. Phytother Res. 27(1):46-53. Assay: Acetylcholine in mouse brain.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
