BioAssay Systems/EnzyChrom™ Ascorbic Acid Assay Kit/100 tests/EASC-100
商品编号：
EASC-100
品牌：
bioassaysys
市场价：
¥7180.00
美元价：
3590.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EnzyChrom™ Ascorbic Acid Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.
Key Features
- Use 20 μL samples. Linear detection range: colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid.
Method
- OD570nm, or FL530/585nm
Samples
- Serum, plasma, urine, saliva, milk, tissue, and cell culture
Species
- All
Size
- 100 tests
Detection Limit
- OD, FL: 6, 1 μM
Shelf Life
- 6 months
More Details
- Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.
We would like to measure ascorbic acid in liver homogenates, which are in 0.25M Sucrose. We want to confirm the sucrose will interfere with the assay?
We have not tested the compatibility of sucrose with this assay. Based on the mechanism of the assay we see no reason why sucrose would interfere and believe the assay will work just fine.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Susanto, L., Y. Siregar, and L. Kusumawati (2018). Vitamin C supplementation improve the sputum conversion culture rate in pulmonary tuberculosis treatment while rifampicin susceptible. IOP Conference Series: Earth and Environmental Science 125(1). Assay: Ascorbic acid in human plasma.Kantar, Michael B., et al (2016). Vitamin variation in Capsicum Spp. provides opportunities to improve nutritional value of human diets. PloS one 11.8: e0161464. Assay: Ascorbic acid in pepper tissue. Trezzi, Jean-Pierre, et al (2016). LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels. Metabolomics 12.6: 96. Assay: Ascorbic acid in human plasma. Sasaki, M. et al (2012). D-2-hydroxyglutarate produced by mutant IDH1 perturbs collagen maturation and basement membrane function. Genes & Dev. 26: 2038-2049. Assay: Ascorbic acid in mouse cell. Ching-Shih Chen et al (2011). Thiazolidinedione Energy Restriction-Mimetic Agents. US 2011/0086895 Al. Assay: Ascorbic acid in mouse cell. To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
