生物测定系统/QuantiChrom&trade；抗氧化剂分析试剂盒/100试验/DTAC-100
商品编号：
DTAC-100
品牌：
bioassaysys
市场价：
¥5580.00
美元价：
2790.00
产品分类：
测序酶
公司分类：
Sequencing_enzyme
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Antioxidant Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism.
Key Features
- Sensitive and accurate. Use 20 μL sample. Linear detection range from 1.5 to 1000 μM Trolox equivalents.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
Method
- OD570nm
Samples
- Serum, plasma, urine, saliva, food and beverage etc
Species
- All
Size
- 100 tests
Detection Limit
- 1.5 μM
Shelf Life
- 6 months
More Details
- An ANTIOXIDANTis a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline. Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery. BioAssay Systems improved assay measures total antioxidant capacity in which Cu2+ is reduced by antioxidant to Cu+. The resulting Cu+ specifically forms a colored complex with a dye reagent. The color intensity at 570nm is proportional to TAC in the sample.
What substances are known to interfere with the antioxidant assay?
Detergents, such as TWEEN-20, TRITON X-100, NP-40, should not be present at any concentration in the test sample. Reducing materials (DTT, 2-mercaptoethanol) and metal chelators (e.g. EDTA) may interfere with the assay.How to determine TAC in tissue samples?
Homogenize tissue samples on ice in cold 1xPBS (~100 mg of tissue/0.5 ml of buffer) and then centrifuge at 12,000g for 15 minutes at 4°C. Remove the supernatant and keep it on ice. For long term storage, store in working aliquots at –80°C.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Indrastiti, R. K., Rahmayanti, F., & Soegyanto, A. I. (2019). Analysis of the Total Antioxidant Capacity of Saliva in Smokers. Pesquisa Brasileira em Odontopediatria e Clinica Integrada, 19(1), 4897. Assay: Antioxidant in human saliva.Moore, M., Moriarty, T. A., Connolly, G., Mermier, C., Amorim, F., Miller, K., & Zuhl, M. (2019). Oral Glutamine Supplement Reduces Subjective Fatigue ratings during Repeated Bouts of Firefighting Simulations. Safety, 5(2), 38. Assay: Antioxidant in horseshoe crab serum. Neves, R. V. P., Rosa, T. S., Souza, M. K., Oliveira, A. J. C., Gomes, G. N. S., Brixi, B. & Prestes, J. (2019). Dynamic, Not Isometric Resistance Training Improves Muscle Inflammation, Oxidative Stress and Hypertrophy in rats. Frontiers in physiology, 10. Assay: Antioxidant in rat muscle tissue. Kumar, A., Singh, K. P., Bali, P., Anwar, S., Kaul, A., Singh, O. P. & Sinha, M. P. (2018). iNOS polymorphism modulates iNOS/NO expression via impaired antioxidant and ROS content in P. vivax and P. falciparum infection. Redox biology, 15, 192-206. Assay: Antioxidant in human serum. Xiao, R. (2018). Characterization and Cultivation of Marine Protist, Thraustochytrium striatum for High-Value Bioproducts. Assay: Antioxidant in mouse cells.Hernandez, O. E. A., Hernandez, L. H. H., Miyasaka, A. S., & Lugo, M. G. (2017). Effects of diets with whole plant-origin proteins added with different ratios of taurine: methionine on the growth, macrophage activity and antioxidant capacity of rainbow trout (Oncorhynchus mykiss) fingerlings. Veterinary and Animal Science, 3, 4-9. Assay: Antioxidant in fish liver tissue.Rishi, P., Arora, S., Kaur, U. J., Chopra, K., & Kaur, I. P. (2017). Better management of alcohol liver disease using a "Microstructured Synbox" System comprising L. plantarum and EGCG. PloS one, 12(1), e0168459. Assay: Antioxidant in rat liver tissue. Villa-Bellosta, R., Hamczyk, M. R., & Andres, V. (2017). Novel phosphate-activated macrophages prevent ectopic calcification by increasing extracellular ATP and pyrophosphate. PloS one, 12(3), e0174998. Assay: Antioxidant in mice marrow. Hasaninasab, A., Esteghamati, A., Azam, K., Yosaee, S., Habibi, N., & Djafarian, K. (2016). Comparison of serum Total Antioxidant Capacity between metabolic syndrome patients and non-metabolic syndrome people with normal or high BMI. Journal of Nutritional Sciences and Dietetics. Assay: Antioxidant in human serum.Subathra, T. A., Pal, G. K., Dhanalakshmi, Y., Nanda, N., & Swaminathan, R. P. (2016). Association of level of cognition with sympathovagal imbalance and cardiovascular risks in prehypertension. International Journal of Clinical and Experimental Physiology, 3(4), 197-203. Assay: Antioxidant in human blood.Hissa, MR et al (2015). A 16-week study to compare the effect of vildagliptin versus gliclazide on postprandial lipoprotein concentrations and oxidative stress in patients with type 2 diabetes inadequately controlled with metformin monotherapy. Diabetol Metab Syndr. 11;7:62. Assay: Antioxidant in human serum. Madrigal-Bujaidar, E et al (2015). Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration. World J Gastroenterology. 21(25): 7718-7729. Assay: Antioxidant in rat liver cells.Madrigal-Santillan, E et al (2015). Hepatoprotective effect of Geranium schiedeanum against ethanol toxicity during liver regeneration. World J Gastroenterology. 21(25): 7718-7729. Assay: Antioxidant in wistar rat liver cells. Hegazi, RM and Kamel HFM (2014). Oxidant Hepatic & /or Haem. Injury on Fuel-StationWorkers Exposed to Benzene Vapor, Possible Protectionof Antioxidants. American Journal of Medicine and Medical Sciences 4(2): 35-46. Assay: Antioxidant in human serum.Ijomone, OM, Olaibi, OK, Nwoha, PU, (2014). Effects of chronic nicotine administration on body weight, food intake and nitric oxide concentration in female and male rats. Pathophysiology. 21:185-190. Assay: Antioxidant in wistar rat serum. Olaibi, OK et al (2014). Histological and Histomorphometric studies of ethanol-injured pylorus and duodenum of Wistar rats pre-treated with Moringa oliefera extract. Al Am een J Med Sci. 7(2):104-111. Assay: Antioxidant in human duodenum tissue. Fawole, O., et al. (2011). Chemical and Phytochemical Properties and Antioxidant Activities of Three Pomegranate Cultivars Grown in South Africa. Food and Bioprocess Technology:1-7. Assay: Antioxidant in plant pomegranate juice.Hadzovi -Dzuvo, A et al (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum. Hadzovi -Dzuvo, A., et al. (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum.Hadzovic-Dzuvo, A et al (2011). Serum total antioxidant capacity in patients with multiple sclerosis. Bosnian Journal of Basic Medical Sciences 11(1):33-36. Assay: Antioxidant in human serum.Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Total Oxidant in human blood. To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
