生物测定系统/QuantiChrom&trade；酸性磷酸酶检测试剂盒/100试验/DACP-100
商品编号：
DACP-100
品牌：
bioassaysys
市场价：
¥2580.00
美元价：
1290.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Acid Phosphatase Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)
Key Features
- Fast and sensitive. Linear detection range (20 μL sample): 0.05 to 60 U/L for a 30 minute reaction.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD405nm
Samples
- Biological (e.g. serum) samples
Species
- All
Size
- 100 tests
Detection Limit
- 0.05 U/L
Shelf Life
- 6 months
More Details
- Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.BioAssay Systems non-radioactive, colorimetric ACP assay is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
I don"t have the correct wavelength filter, will the assay still work?
Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.I don"t have a 96-well plate, will the assay kit work in a 384 well plate, cuvette, etc?
Yes, the assay can be used in any standard plate and cuvette. Simply adjust the total reaction volume to your container. For 384 well use 50 μL, for a cuvette use 500 μL or 1 mL.Does the assay kit work in particular species?
Yes, this assay works in all species.How do I prepare cell or tissue samples for assays?
Please refer to the protocol for sample preparation:Blood samples: Serum and plasma should be diluted 2-5 fold.Tissue: Prior to dissection, rinse tissue in Tris buffered saline (pH 7.4) to remove blood. Homogenize tissue (50 mg) in 200 μL 50 mM Tris buffer (pH 7.5). Centrifuge at 14,000 x g for 10 min at 4°C. Remove supernatant for assay. Cell lysate: Collect cells by centrifugation at 2,000 x g for 5 min at 4°C. For adherent cells, do not harvest cells using proteolytic enzymes; rather use a rubber policeman. Homogenize or sonicate cells in an appropriate volume of cold 50 mM Tris buffer (pH 7.5), approximately one million cells per mL. Centrifuge at 14,000 x g for 10 min at 4°C. Remove supernatant for assay. All samples can be stored at -80 to -20°C for at least one month.Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Since Assay Buffer is very light sensitive, always keeps buffer in the dark (amber bottle or tube) as much as possible for long term usage.Do I need to use a standard or standard curve with each assay run?
Yes, it is highly recommended.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions. No citations for this new product. Please check back later.You mayclick here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
