生物测定系统/QuantiChrom&trade；乙酰胆碱酯酶抑制剂筛选试剂盒/100试验/IACE-100
商品编号：
IACE-100
品牌：
bioassaysys
市场价：
¥6380.00
美元价：
3190.00
产品分类：
影像显示
公司分类：
OLED_Controller
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QuantiChrom™ Acetylcholinesterase Inhibitor Screening Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- HTS for inhibitor screening and evaluation of acetylcholinesterase inhibitors.
Key Features
- Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at room temperature.
- High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD412nm
Samples
- Acetylcholinesterase
Species
- All
Size
- 100 tests
Shelf Life
- 12 months
More Details
- ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Inhibition of the enzyme leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. AChE inhibition is an important target for the management of Alzheimer"s disease. In addition to Alzheimer"s disease, AChE inhibitors have been useful in the diagnosis or treatment of diseases such as glaucoma, myasthenia gravis, bladder distention, and more. BioAssay Systems QuantiChrom™ Acetylcholinesterase Inhibitor Assay is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5"-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412nm, is proportionate to the enzyme activity in the sample.
Does the kit come with AChE or a control AChE inhibitor?
No, these are not included.How do I store the kit?
This kit is shipped at room temperature. Upon receiving, please the kit at -20°C. The assay buffer can be stored at room temperature if desired.What species of Acetylcholinesterase was the kit optimized for?
The kit was optimized for AChE for E. electricus (electric eel).What if I am interested in testing inhibition of a species that is not electriceel?
We recommend that you experimentally determine the Km and then adjust the volume of substrate in the Working Reagent so that the final concentration of the substrate in the 200µL Reaction Volume is near the Km.What Acetylcholinesterase inhibitors were tested using this kit?
Physostigmine (CAS Number 57-47-6) and Donepezil (CAS Number 120014-06-4).For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.El-Malah, A., Abouelatta, A. I., Mahmoud, Z., & Salem, H. H. (2019). New cyclooctathienopyridine derivatives in the aim of discovering better Anti-Alzheimer"s agents. Journal of Molecular Structure. Assay: Acetylcholinesterase inhibitor in brain cholinesterase.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
品牌介绍
BioAssay Systems位于加州北部，是一家专注于提供高质量试剂盒的生物技术公司，试剂盒种类广泛、使用简便、操作简单、快速且性能优越，用户不需花额外的时间来作调适测试。BioAssay Systems产品具有快速、简单、高通量（可用于HTS药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征，且采用发光、生物发光、化学发光、荧光等非放射性的检测技术，安全无害可靠。
